First Clinical Cases of KPC-2-Producing Klebsiella pneumoniae ST258 in Algeria and Outbreak of Klebsiella pneumoniae ST101 Harboring blaOXA-48 Gene in the Urology Department of Annaba Hospital.
Identifieur interne : 000724 ( Main/Exploration ); précédent : 000723; suivant : 000725First Clinical Cases of KPC-2-Producing Klebsiella pneumoniae ST258 in Algeria and Outbreak of Klebsiella pneumoniae ST101 Harboring blaOXA-48 Gene in the Urology Department of Annaba Hospital.
Auteurs : Safa Brahmia [Algérie, France] ; Rym Lalaoui [France] ; Sabrina Nedjai [Algérie] ; Nassima Djahmi [Algérie] ; Samir Chettibi [Algérie] ; Jean-Marc Rolain [France] ; Sofiane Bakour [France]Source :
- Microbial drug resistance (Larchmont, N.Y.) [ 1931-8448 ] ; 2021.
Abstract
Objectives: The aim of this study was to characterize the molecular mechanisms of carbapenem resistance in Klebsiella pneumoniae isolated from the urology department of Annaba hospital, Algeria. Methods: Between January 2015 and September 2017, 14 carbapenem-resistant K. pneumoniae strains were isolated during routine surveillance work at Ibn Roched hospital of Annaba, Algeria, from the urology department. Theses strains were recovered, and carbapenem resistance mechanisms were investigated. The strains were identified by using matrix-assisted laser desorption and ionization time-of-flight mass spectrometry. Antibiotic susceptibility was assessed by using the Kirby-Bauer method, whereas minimum inhibitory concentration of imipenem/ertapenem and colistin was determined by Etest and broth microdilution methods, respectively. Carbapenem resistance determinants were studied by using PCR and sequencing methods and analyzed by BLAST against the Antibiotic Resistance Gene-ANNOTation (ARG-ANNOT) database. Clonal relationship of strains was performed by using multilocus sequence typing (MLST). Transferability of carbapenem resistance genes was assessed by conjugation and transformation experiments. Results: Fourteen carbapenem-resistant K. pneumoniae isolates were found to be resistant to the eight β-lactam antibiotics tested (except to imipenem for two isolates). Carbapenemase production was positive for all isolates. Molecular characterization revealed that blaKPC-2 and blaOXA-48 genes were detected in 3 (21.4%) and 11 isolates (78.6%), respectively. Other β-lactamases genes were identified, including blaCTX-M-15, blaSHV-1-or 12, and blaTEM-1. MLST revealed that the 14 isolates belonged to 2 different sequence types (STs), including ST101 (11 OXA-48-producing K. pneumoniae) and ST258 (3 KPC-2-producing K. pneumoniae). PCR amplifications for blaKPC-2 and blaOXA-48 carbapenemases genes performed on extracted plasmids, showed positive results, suggesting that both carbapenemase genes were probably borne by plasmids. Conclusion: We report here the first identification of KPC-2-producing K. pneumoniae ST258 in Algerian hospitals and an outbreak of OXA-48-producing K. pneumoniae isolates ST101 in the urology department of Ibn Roched hospital located in Annaba, Algeria.
DOI: 10.1089/mdr.2020.0080
PubMed: 32991248
Affiliations:
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last="Chettibi">Samir Chettibi</name><affiliation wicri:level="1"><nlm:affiliation>Service de Chirurgie Urologique, Transplantation, CHU d'Annaba, Annaba, Algérie.</nlm:affiliation><country xml:lang="fr">Algérie</country><wicri:regionArea>Service de Chirurgie Urologique, Transplantation, CHU d'Annaba, Annaba</wicri:regionArea><wicri:noRegion>Annaba</wicri:noRegion></affiliation></author><author><name sortKey="Rolain, Jean Marc" sort="Rolain, Jean Marc" uniqKey="Rolain J" first="Jean-Marc" last="Rolain">Jean-Marc Rolain</name><affiliation wicri:level="3"><nlm:affiliation>Aix Marseille Univ, IRD, APHM, MEPHI, Marseille, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Aix Marseille Univ, IRD, APHM, MEPHI, Marseille</wicri:regionArea><placeName><region type="region">Provence-Alpes-Côte d'Azur</region><region type="old region">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation><affiliation 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xml:lang="fr">Algérie</country><wicri:regionArea>Laboratoire de Biochimie et de Microbiologie Appliquée, Département de Biochimie, Université Badji Mokhtar-Annaba, Annaba</wicri:regionArea><wicri:noRegion>Annaba</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:affiliation>Aix Marseille Univ, IRD, APHM, MEPHI, Marseille, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Aix Marseille Univ, IRD, APHM, MEPHI, Marseille</wicri:regionArea><placeName><region type="region">Provence-Alpes-Côte d'Azur</region><region type="old region">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>IHU-Méditerranée Infection, Marseille, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>IHU-Méditerranée Infection, Marseille</wicri:regionArea><placeName><region type="region">Provence-Alpes-Côte d'Azur</region><region type="old region">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Lalaoui, Rym" sort="Lalaoui, Rym" uniqKey="Lalaoui R" first="Rym" last="Lalaoui">Rym Lalaoui</name><affiliation wicri:level="3"><nlm:affiliation>Aix Marseille Univ, IRD, APHM, MEPHI, Marseille, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Aix Marseille Univ, IRD, APHM, MEPHI, Marseille</wicri:regionArea><placeName><region type="region">Provence-Alpes-Côte d'Azur</region><region type="old region">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>IHU-Méditerranée Infection, Marseille, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>IHU-Méditerranée Infection, Marseille</wicri:regionArea><placeName><region type="region">Provence-Alpes-Côte d'Azur</region><region type="old region">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Nedjai, Sabrina" sort="Nedjai, Sabrina" uniqKey="Nedjai S" first="Sabrina" last="Nedjai">Sabrina Nedjai</name><affiliation wicri:level="1"><nlm:affiliation>Laboratoire de Microbiologie Central, CHU Dorban, Faculté de Médecine, Université Badji Mokhtar-Annaba, Annaba, Algérie.</nlm:affiliation><country xml:lang="fr">Algérie</country><wicri:regionArea>Laboratoire de Microbiologie Central, CHU Dorban, Faculté de Médecine, Université Badji Mokhtar-Annaba, Annaba</wicri:regionArea><wicri:noRegion>Annaba</wicri:noRegion></affiliation></author><author><name sortKey="Djahmi, Nassima" sort="Djahmi, Nassima" uniqKey="Djahmi N" first="Nassima" last="Djahmi">Nassima Djahmi</name><affiliation wicri:level="1"><nlm:affiliation>Laboratoire de Microbiologie Central, CHU Dorban, Faculté de Médecine, Université Badji Mokhtar-Annaba, Annaba, Algérie.</nlm:affiliation><country xml:lang="fr">Algérie</country><wicri:regionArea>Laboratoire de Microbiologie Central, CHU Dorban, Faculté de Médecine, Université Badji Mokhtar-Annaba, Annaba</wicri:regionArea><wicri:noRegion>Annaba</wicri:noRegion></affiliation></author><author><name sortKey="Chettibi, Samir" sort="Chettibi, Samir" uniqKey="Chettibi S" first="Samir" last="Chettibi">Samir Chettibi</name><affiliation wicri:level="1"><nlm:affiliation>Service de Chirurgie Urologique, Transplantation, CHU d'Annaba, Annaba, Algérie.</nlm:affiliation><country xml:lang="fr">Algérie</country><wicri:regionArea>Service de Chirurgie Urologique, Transplantation, CHU d'Annaba, Annaba</wicri:regionArea><wicri:noRegion>Annaba</wicri:noRegion></affiliation></author><author><name sortKey="Rolain, Jean Marc" sort="Rolain, Jean Marc" uniqKey="Rolain J" first="Jean-Marc" last="Rolain">Jean-Marc Rolain</name><affiliation wicri:level="3"><nlm:affiliation>Aix Marseille Univ, IRD, APHM, MEPHI, Marseille, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Aix Marseille Univ, IRD, APHM, MEPHI, Marseille</wicri:regionArea><placeName><region type="region">Provence-Alpes-Côte d'Azur</region><region type="old region">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>IHU-Méditerranée Infection, Marseille, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>IHU-Méditerranée Infection, Marseille</wicri:regionArea><placeName><region type="region">Provence-Alpes-Côte d'Azur</region><region type="old region">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Bakour, Sofiane" sort="Bakour, Sofiane" uniqKey="Bakour S" first="Sofiane" last="Bakour">Sofiane Bakour</name><affiliation wicri:level="3"><nlm:affiliation>Aix Marseille Univ, IRD, APHM, MEPHI, Marseille, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Aix Marseille Univ, IRD, APHM, MEPHI, Marseille</wicri:regionArea><placeName><region type="region">Provence-Alpes-Côte d'Azur</region><region type="old region">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>IHU-Méditerranée Infection, Marseille, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>IHU-Méditerranée Infection, Marseille</wicri:regionArea><placeName><region type="region">Provence-Alpes-Côte d'Azur</region><region type="old region">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author></analytic><series><title level="j">Microbial drug resistance (Larchmont, N.Y.)</title><idno type="eISSN">1931-8448</idno><imprint><date when="2021" type="published">2021</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><b><i>Objectives:</i></b> The aim of this study was to characterize the molecular mechanisms of carbapenem resistance in <i>Klebsiella pneumoniae</i> isolated from the urology department of Annaba hospital, Algeria. <b><i>Methods:</i></b> Between January 2015 and September 2017, 14 carbapenem-resistant <i>K. pneumoniae</i> strains were isolated during routine surveillance work at Ibn Roched hospital of Annaba, Algeria, from the urology department. Theses strains were recovered, and carbapenem resistance mechanisms were investigated. The strains were identified by using matrix-assisted laser desorption and ionization time-of-flight mass spectrometry. Antibiotic susceptibility was assessed by using the Kirby-Bauer method, whereas minimum inhibitory concentration of imipenem/ertapenem and colistin was determined by Etest and broth microdilution methods, respectively. Carbapenem resistance determinants were studied by using PCR and sequencing methods and analyzed by BLAST against the Antibiotic Resistance Gene-ANNOTation (ARG-ANNOT) database. Clonal relationship of strains was performed by using multilocus sequence typing (MLST). Transferability of carbapenem resistance genes was assessed by conjugation and transformation experiments. <b><i>Results:</i></b> Fourteen carbapenem-resistant <i>K. pneumoniae</i> isolates were found to be resistant to the eight β-lactam antibiotics tested (except to imipenem for two isolates). Carbapenemase production was positive for all isolates. Molecular characterization revealed that <i>bla</i><sub>KPC-2</sub> and <i>bla</i><sub>OXA-48</sub> genes were detected in 3 (21.4%) and 11 isolates (78.6%), respectively. Other β-lactamases genes were identified, including <i>bla</i><sub>CTX-M-15</sub>, <i>bla</i><sub>SHV-1-or 12</sub>, and <i>bla</i><sub>TEM-1</sub>. MLST revealed that the 14 isolates belonged to 2 different sequence types (STs), including ST101 (11 OXA-48-producing <i>K. pneumoniae</i>) and ST258 (3 KPC-2-producing <i>K. pneumoniae</i>). PCR amplifications for <i>bla</i><sub>KPC-2</sub> and <i>bla</i><sub>OXA-48</sub> carbapenemases genes performed on extracted plasmids, showed positive results, suggesting that both carbapenemase genes were probably borne by plasmids. <b><i>Conclusion:</i></b> We report here the first identification of KPC-2-producing <i>K. pneumoniae</i> ST258 in Algerian hospitals and an outbreak of OXA-48-producing <i>K. pneumoniae</i> isolates ST101 in the urology department of Ibn Roched hospital located in Annaba, Algeria.</div></front></TEI><affiliations><list><country><li>Algérie</li><li>France</li></country><region><li>Provence-Alpes-Côte d'Azur</li></region><settlement><li>Marseille</li></settlement></list><tree><country name="Algérie"><noRegion><name sortKey="Brahmia, Safa" sort="Brahmia, Safa" uniqKey="Brahmia S" first="Safa" last="Brahmia">Safa Brahmia</name></noRegion><name sortKey="Chettibi, Samir" sort="Chettibi, Samir" uniqKey="Chettibi S" first="Samir" last="Chettibi">Samir Chettibi</name><name sortKey="Djahmi, Nassima" sort="Djahmi, Nassima" uniqKey="Djahmi N" first="Nassima" last="Djahmi">Nassima Djahmi</name><name sortKey="Nedjai, Sabrina" sort="Nedjai, Sabrina" uniqKey="Nedjai S" first="Sabrina" last="Nedjai">Sabrina Nedjai</name></country><country name="France"><region name="Provence-Alpes-Côte d'Azur"><name sortKey="Brahmia, Safa" sort="Brahmia, Safa" uniqKey="Brahmia S" first="Safa" last="Brahmia">Safa Brahmia</name></region><name sortKey="Bakour, Sofiane" sort="Bakour, Sofiane" uniqKey="Bakour S" first="Sofiane" last="Bakour">Sofiane Bakour</name><name sortKey="Bakour, Sofiane" sort="Bakour, Sofiane" uniqKey="Bakour S" first="Sofiane" last="Bakour">Sofiane Bakour</name><name sortKey="Brahmia, Safa" sort="Brahmia, Safa" uniqKey="Brahmia S" first="Safa" last="Brahmia">Safa Brahmia</name><name sortKey="Lalaoui, Rym" sort="Lalaoui, Rym" uniqKey="Lalaoui R" first="Rym" last="Lalaoui">Rym Lalaoui</name><name sortKey="Lalaoui, Rym" sort="Lalaoui, Rym" uniqKey="Lalaoui R" first="Rym" last="Lalaoui">Rym Lalaoui</name><name sortKey="Rolain, Jean Marc" sort="Rolain, Jean Marc" uniqKey="Rolain J" first="Jean-Marc" last="Rolain">Jean-Marc Rolain</name><name sortKey="Rolain, Jean Marc" sort="Rolain, Jean Marc" uniqKey="Rolain J" first="Jean-Marc" last="Rolain">Jean-Marc Rolain</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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